A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.
نویسنده
چکیده
The synthesis and evaluation of a new detergent that is a zwitterionic derivative of cholic acid is presented. This detergent combines the useful properties of both the sulfobetaine-type detergents and the bile salt anions. The new detergent proved to be effective at solubilizing membrane proteins in a nondenatured state.
منابع مشابه
Thermodynamic investigation of micellization of zwitterionic detergent CHAPS: temperature, salt and pH dependence
The zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS), derivative of naturally occurring bile salts, is widely used in biochemistry for membrane protein and receptor solubilization and purification. Since the efficiency of protein stabilization is known to be highly related to the detergent concentration, it is very important for the researcher to predic...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 77 11 شماره
صفحات -
تاریخ انتشار 1980